Cytotoxic and osteogenic property of trilaurin assisted hydroxyapatite nanorods in simulated body fluid environment

Abstract

In this research work, the cytogenic and osteogenic properties of biomimetically synthesised nanohydroxyapatite (nanoHAP) powders using Trilaurin (TL), as a surfactant was attempted. Microwave (MW) and ultrasound (US) technologies were also employed to enhance the efficacy of the method. Different HAP samples were prepared using 1.5 times concentrated Simulated Body Fluid (SBF) with 1, 5 and 15 days immersion inorder to achieve pure nanohydroxyapatite powders. The formation of HAP was well supported by FTIR and Raman spectroscopy, which shows no impurity peaks. By increasing the time of soaking in SBF, growth, size and crystallinity of HAP were found to decrease as evidenced by XRD data. HRSEM studies reveal that nano-plates and rod shaped nano HAP could be obtained by this method. So the obtained TL assisted HAP exhibits high thermal stability as confirmed by TGA analysis. The invitro cytotoxicity was evaluated by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay using human osteoblastic cell line (MG-63) and Michigan Cancer Foundation-7 (MCF-7) breast cancer cell line. The obtained result supports the osteoblast property and less toxicity. Inorder to further prove the osteoblast property, trypan blue exclusion test (live dead assay) were carried out using MG-63 cell line and the obtained results favours the bone forming ability.