Cytotoxic and genotoxic effects of Br-containing oxaphosphole on Allium cepa L. root tip cells and mouse bone marrow cells

Vanya P Kalcheva,Asya P Dragoeva,Karamfil N Kalchev,Dobromir D Enchev

doi:10.1590/s1415-47572009000200028

Vanya P Kalcheva, Asya P Dragoeva + Show 2 more

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https://doi.org/10.1590/s1415-47572009000200028

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Journal: Genetics and Molecular Biology	Publication Date: Jan 1, 2009
Citations: 29	License type: cc-by

Affiliation: Shumen University

Abstract

The continuous production and release of chemicals into the environment has led to the need to assess their genotoxicity. Numerous organophosphorus compounds with different structures have been synthesized in recent years, and several oxaphosphole derivatives are known to possess biological activity. Such chemical compounds may influence proliferating cells and cause disturbances of the genetic material. In this study, we examined the cytotoxicity and genotoxicity of 4-bromo-N,N-diethyl-5,5-dimethyl-2,5-dihydro-1,2-oxaphosphol-2-amine 2-oxide (Br-oxph). In A. cepa cells, Br-oxph (10-9 M, 10 -6 M and 10 -3 M) reduced the mitotic index 48 h after treatment with the two highest concentrations, with no significant effect at earlier intervals. Mitotic cells showed abnormalities 24 h and 48 h after treatment with the two lowest concentrations but there were no consistent changes in interphase cells. Bone marrow cells from mice treated with Br-oxph (2.82 x 10 -3 μg/kg) also showed a reduced mitotic index after 48 h and a greater percentage of cells with aberrations (principally chromatid and isochromatid breaks). These findings indicate the cytotoxicity and genotoxicity of Br-oxph in the two systems studied.

Highlights

The chemistry of organophosphorus compounds is a subject of increasing interest, and a large number of compounds with different structures, properties and biological activites have been synthesized (Smee and Reist, 1996; Leblond et al, 2002)
Br-oxph was synthesized in the Laboratory of Organic Chemistry of the University of Shumen (Bulgaria) (Angelov and Enchev, 1987)
Since Enchev et al (1986) showed that some oxaphospholes affected plant growth at concentrations of 10-9 M, 10-6 M and 10-3 M, these concentrations were used in the Allium test

Summary

Introduction

The chemistry of organophosphorus compounds is a subject of increasing interest, and a large number of compounds with different structures, properties and biological activites have been synthesized (Smee and Reist, 1996; Leblond et al, 2002). Chromosomal aberrations in Allium root cells were assessed by light microscopy (Rank, 2003).

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