Abstract
In this work, the biological and chemical fingerprints of three extracts (ethyl acetate, methanol, and water) from two Potentilla species (Potentilla reptans and P. speciosa) were investigated. Antioxidant, enzyme inhibitory, and cytotoxic activities were performed for the biological fingerprint. For the chemical characterization, total bioactive components, and individual phenolic components were determined using photometric and HPLC methods, respectively. The main identified phenolic compounds in these extracts were rutin and catechin. Methanol and water extracts contained the highest total phenolic and flavonoid content. The results of antioxidant assays showed that methanol and water extracts displayed higher antioxidant activity compared to the ethyl acetate extract. Generally, methanol and water extracts exhibited higher biological activities correlated with higher levels the bioactive components. For P. speciosa, the methanol extract exhibited the highest enzyme inhibitory activity (except BChE inhibitory activity). P. reptans exhibited also high antiproliferative activity against MCF-7 cells whilst P. speciosa had weak to moderate activity against both of A549 and MCF-7 cell lines. The results suggest that Potentilla species could be potential candidates for developing new phyto-pharmaceuticals and functional ingredients.
Highlights
Natural products and functional-food ingredients gained interest due to their valuable biological effects including antioxidant, anticancer or antimicrobial
Tyrosinase is a key enzyme in melanin biosynthesis and the inhibition of this enzyme is associated with the prevention of skin disorders (SD) (Schelterns and Feldman, 2003)
To understand the relationship between antioxidant capacity and phenolic components, the phenolic components of two Potentilla species were determined by using HPLC-PDA (Figure 1)
Summary
Natural products and functional-food ingredients gained interest due to their valuable biological effects including antioxidant, anticancer or antimicrobial. Studies on natural products and medicinal plants are one of the most important subjects in pharmaceutical area (Sut et al, 2016). Many researches have reported that synthetic inhibitors have unfavorable effects such as nausea and diarrhea (Nouri et al, 2014; Anantharaman et al, 2016; Bekir et al, 2016). Due to these adverse effects, there is an increasing search for inhibitors derived from natural products (non-toxic and effective) against key enzymes related with these diseases (Day, 1998; Qin et al, 2013)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
