Abstract
Aim: The present study aimed to analyse the cytotoxicity of zinc oxide nanoparticles (ZnO NPs) that are biosynthesized using extract of Abies webbiana
 Methodology: Zinc oxide nanoparticles were synthesized by green approach using A. webbiana and were characterized by UV- vis spectroscopy. The powdered A. webbiana mediated ZnO NPs were subjected to cytotoxic tests for different concentrations by brine shrimp lethality assay.
 Results: ZnO NPs synthesized from A. webbiana showed reduced toxicity at lower concentrations. After 48 hours, it was found that at a minimal concentration of 5 µl, 90% of the nauplii and in concentration of 10 µl and 20 µl, 80% of the nauplii were alive. At a concentration of 40 µl and 80 µl, 70% of the nauplii were alive. Whereas the control showed 100% of the nauplii to be alive.
 Conclusion: Various nanoformulations of A. webbiana mediated ZnO NPs can be developed at optimal concentrations for therapeutics that are safe and economical.
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