Cytotoxic Activity of Bark of Oroxylon indicum Benth against U937 and K562 Human Leukemic Cell Lines

Abstract

Cancer, a leading cause of death, is a condition where cells have lost control over their mode of growth. We evaluated the anti- proliferative and cytotoxic and apoptogenic activity of methanolic extract of Oroxylon indicum Bark (MEOIB) on U937, K562 cells and normal lymphocytes. The cell viability was studied by MTT assay. Cell morphology was determined by Fluorescence, Confocal microscope and DNA fragmentation by agarose gel electrophoresis study. MEOIB inhibited the viability of cells in a time & concentration dependent manner. The Morphologically it was found that externalization of PS from inner leaflet to outer leaflet of the cell membrane and condensed chromatin, membrane blabbing, fragmentation of nuclei in all the treated cells than control and DNA bands appeared in the form of ladder after treatment with MEOIB. The present study reveals that the MEOIB possesses potent anti-leukemic activity against U937 and K562 cell line but there no toxicity in normal lymphocytes.