Abstract
Porodaedalea pini is a medicinally important mushroom with antioxidant, cytotoxic, immunostimulating, antitumor, antiviral and immunomodulating activities. Therefore, in this study, P. pini methanol extract and isolated compounds from the methanol extract were tested for cytotoxic activities against DLD-1 (colorectal cancer) and CCD-18Co (human colon fibroblast cell line) by using Alamar Blue assay. Cytotoxic activity on DLD-1 was decreased in the order of P. pini methanol extract> 4-(3,4-dihydroxyphenyl)but-3-en-2-one (3)> pinoresinol (2)> ergosta-7,24(28)-dien-3β-ol (1). P. pini methanol extract was determined to have the best cytotoxic activity with the lowest IC50 value on DLD-1 (IC50: 25.33±0.29 µg/mL) and the highest IC50 value on CCD-18Co (434.30±1.45 µg/mL). Within the scope of the findings, it is thought that P. pini mushroom can be used as a new and natural agent in the treatment of colorectal cancer.
Highlights
Cancer ranks second in deadly diseases after cardiovascular diseases worldwide
Cytotoxic activities against DLD-1 and CCD-18Co of P. pini methanol extract and ergosta-7,24(28)-dien-3β-ol (1), pinoresinol (2) and 4-(3,4-dihydroxyphenyl)but-3-en-2-one (3) compounds isolated from the methanol extract were tested by Alamar Blue assay
Chemotherapy, cytotoxic drugs, radiotherapy, and surgery are the main methods used in the treatment of colorectal cancer
Summary
Cancer ranks second in deadly diseases after cardiovascular diseases worldwide. Approximately 10% of diagnosed cancers or cancer-related deaths each year consist of colorectal cancer (Dekker et al, 2019). In Chinese medicine, Phellinus genus has been reported to be used in the treatment of many diseases such as arthritis of the knee, stomachaches, tumors, inflammation, lymphatic and gastroenteric disorders and to promote longevity in Chinese medicine (Ganeshpurkar et al, 2010; Seephonkai et al, 2011) Bioactive compounds such as sesquiterpenes, triterpenes, steroids, pigments, and polysaccharides, responsible for estrogenic, antiviral, antioxidant, antitumor and anti-estrogenic activity have been purified from Phellinus species (Wu et al, 2010; Song et al, 2014; Wang et al, 2014; Pei et al, 2015). Antioxidant, antifungal, anticholinesterase, anti-tyrosinase, cytotoxic activities, and inhibition effects on NO production in murine macrophages-RAW 264.7 cells by the compounds isolated from P. pini have been revealed by previous studies (Ayer et al, 1996; Wangun & Hertweck, 2007; Jang &Yang, 2011; Deveci et al, 2019a). The study was aimed to evaluate cytotoxic activities against DLD-1 (colorectal cancer) and CCD-18Co (human colon fibroblast cell line) of both P. pini methanol extract and ergosta-7,24(28)-dien-3β-ol, pinoresinol, and 4-(3,4dihydroxyphenyl)but-3-en-2-one compounds isolated from the methanol extract in our previous study (Deveci et al, 2019a)
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