Cytotoxic Activities of Isolated Compounds from Prangos uechtritzii Boiss & Hausskn

Abstract

Prangos uechtritzii Boiss&Hausskn is an endemic plant of Türkiye, and the plant roots are rich in coumarins. This study aims to evaluate the cytotoxic activities of the 16 natural compounds along with the n-hexane (PH), chloroform (PC), and methanol (PM) extracts of P. uechtritzii. For this purpose, fourteen coumarin derivatives; umbelliferone(1), 6-formylumbelliferone(2), suberosin(3), 7-demethylsuberosin(4), (+)-ulopterol(5), tamarin(6), psoralen(7), imperatorin(8), (+)-oxypeucedanin(9), (+)-oxypeucedanin hydrate(10), (+)-oxypeucedanin methanolate(11), (+)-marmesin(12), (-)-prantschimgin(13), and (-)-adicardin(14); two polyacetylenes (-)-panaxynol(15), (+)-falcarindiol(16) and three extracts (PH, PC, and PM) were tested for their cytotoxicity against two healthy (HK-2, NIH/3T3), and four cancer (MCF-7, A-549, SH-SY5Y, PC-3) cells by WST-1 method. Doxorubicin was used as a positive control. PH and PC showed cytotoxic effects on all the cell lines with IC50 values of 8.16-91.56 μg/mL. PH displayed a selective effect on SH-SY5Y cells [Selectivity Index (SI)= 2.5] compared to NIH/3T3. PC exhibited cytotoxic effects on PC-3 cells (SI=2) compared to both NIH/3T3, and HK-2. PM didn’t display cytotoxicity at 100 μg/mL. (-)-Panaxynol, (+)-falcarindiol, 6-formylumbelliferone, 7-demethylsuberosin, and suberosin exhibited effects with IC50 values of 8.65-87.91 μM, while others didn’t work at 100 μM. So, P. uechtritzii could be a promising natural source in the development of new drugs for cancer treatment.