Abstract
To confirm whether or not cytosolic NADPH-UQ reductase is involved in the recycling of cellular ubiquinol (UQH 2) consumed during lipid peroxidation, the effect of a UQ-10 supplement on the NADPH-UQ reductase and cellular defense against oxidative damage in rat livers was investigated. Supplements of UQ-10 for 14 days enhanced the levels of UQH 2-10 and NADPH-UQ reductase in rat livers without any appreciable changes in other antioxidant contents and related enzyme activities. However, the injection of carbon tetrachloride (CCl 4) into the rats induced lipid peroxidation and decreased the cellular UQH 2-10 contents (and increased equivalent amounts of UQ-10), as well as decreasing the ascorbic acid, reduced glutathione (GSH) and α-tocopherol contents of the rat livers. Administration of the UQ-10 supplement prior to the CCl 4 treatment spared α-tocopherol (but not GSH or ascorbic acid), inhibited lipid peroxidation, and thus improved CCl 4-induced hepatitis. These findings support the notion that NADPH-UQ reductase in cytosol is the enzyme responsible for the regeneration of UQH 2 from UQ formed by lipid peroxidation in cells.
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