Abstract
We used the whole-cell patch-clamp technique to study the secretory activity of single cells by monitoring changes in membrane capacitance [Neher, E. and Marty, A. (1982) Proc. Natl. Acad. Sci. USA 79, 523–535] in anterior pituitary cells. Unexpectedly we have observed that increasing intracellular chloride ions stimulate Ca 2+-induced exocytosis in a dose-dopendent fashion ( K d = 12 mM). These results demonstrate a role of cytosolic chloride ions in the regulation of exocytotic secretion in anterior pituitary cells. It is suggested that chloride channels, in addition to playing a part in regulating membrane electrical activity [Korn, S.J., Bolden, A. and Horn, A. (1991) J. Physiol. 439, 423–437; Penner, R., Matthews, G. and Horn, A. (1988) Nature 334, 499–504] and cytosolic pH [Kaila, K. and Voipio, J. (1987) Nature 330, 163–165], are also involved in the modulation of cytosolic chloride concentration and thus in the control of exocytosis.
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