Abstract
Hypophysectomized male rats were injected with either ACTH or saline (control) and killed 15 min later. Mitochondrial and 235, 000 x g supernatant (cytosol) fractions were prepared from the adrenal glands. When cytosol from ACTH-treated animals was mixed with isolated mitochondria from the control animals, a dose-dependent increase in pregnenolone production occurred. Liver cytosol caused no increase in the production of pregnenolone. Thus, ACTH elicits a soluble adrenal factor(s) which activates the mitochondrial cholesterol side-chain cleavage system which is the rate-limiting step in steroidogenesis. The cytosol stimulatory factor was found to be nondialyzable, heat-sensitive, and resistant to trypsinization.
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