Cytoskeletal organization and tissue patterns of epithelia in the sponge Ephydatia mülleri.

Abstract

Comparative morphological studies on cytoskeletal patterns of sponge basal epithelium at the tissue level have been performed by diverse methods, including immunofluorescence microscopy, and scanning and transmission electron microscopy of stained whole mounts, thin sections or replicas. These methods give results consistent with each other and show the importance of actin assemblies, which function in addition to the microtubular system and in the absence of intermediate filaments. Actin microfilaments indeed are involved in the formation of cables and networks closely associated with the plasma membrane. Both the cables and the networks result from arrangements of microfilaments into bundles of variable size, and the two types of assembly are probably interconvertible. Microfilaments appear to be implicated in the establishment of spot desmosomes and as devices for cell-to-substratum attachment. Due to the desmosomal articulations from cell to cell, the actin cytoskeleton is framed throughout the complete epithelium. It supports the unitary nature of the entire tissue, which is constructed and functions as a whole. It therefore establishes the "histoskeleton" of basic epithelial tissues. The histoskeleton is involved in all epithelial activities but is not uniformly organized into identical cell patterns at the tissue level because activities are sequential and not synchronized in all cells. Similar cytoskeletal patterns exist only in groups of cells, and this suggests that, at a given time, the multiple functions of the epithelium may be mediated by the occurrence of several multicellular functional units within a single epithelial tissue.