Cytoprotective effect of a functional antipollutant blend through reducing B[a] P-induced intracellular oxidative stress and UVA exposure.

Gizem Örs,Sultan Gülçe İz

doi:10.3906/biy-1802-43

Abstract

Benzo[a]pyrene (B[a]P) is a ubiquitous environmental pollutant that reacts with skin and induces intracellular oxidative stress through reactive oxygen species (ROS) accumulation. The antipollution properties of natural extracts, especially including antioxidants, for inhibiting ROS in cells are gaining importance, in addition to the anticancer effects attributed to them. In this study, a commercial functional antipollutant blend of plant extracts consisting of ellagic acid standardized Punica granatum peel extract, Sambucus nigra fruit extract, Prunus cerasus seed extract, and hydrolyzed wheat protein with high antioxidant properties and UV damage-protective properties attributed to each one was investigated. The cytoprotective effect of this functional antipollutant blend was determined by ROS assay through reducing the level of intracellular ROS induced by B[a]P as an oxidative stress factor in human neonatal keratinocytes and fibroblast cells. In addition, the cytoprotective effect of the functional antipollutant blend after UVA exposure was also determined. It is shown that the oxidative damage induced by B[a]P and UVA, which are the most abundant factors of chemical and physical pollution, would be prevented by the functional antipollutant blend. Thus, it can be concluded that this antipollutant functional blend may offer a promising ingredient for the cosmetic industry's skincare products.

Highlights

Environmental factors such as temperature, climate, dryness, heavy metals, air pollution, and exposure to sunlight (ultraviolet (UV) radiation) directly influence skin and hair health (Baudouin et al, 2002; López-Alarcón et al, 2013)
There was not a significant difference between the cell lines in the means of cytotoxicity (P > 0.05). Both HS2 and L929 cells at 0.75 mg/mL and 0.375 mg/ mL concentrations showed significant decreases in cell viability compared to controls (P < 0.001)
As the cell viability was inversely correlated with the concentration of the antipollutant plant extract, 0.09375 mg/mL (≈0.1 mg/mL) concentration, which provides over 90% cell viability both for HS2 and L929, was chosen to be used in other experimental sets to determine the cytoprotective activity after B[a]P or UVA treatment

Summary

Introduction

Environmental factors such as temperature, climate, dryness (reduction of ambient temperature), heavy metals, air pollution (including mainly toxic gases), and exposure to sunlight (ultraviolet (UV) radiation) directly influence skin and hair health (Baudouin et al, 2002; López-Alarcón et al, 2013). Determination of antipollution effect of the natural plant extract after B[a]P and UVA exposure B[a]P is an environmental pollutant used to induce intracellular oxidative stress (Zhang et al, 2004) and genotoxicity (Gao et al, 2005) for in vitro experimental models.

Results

Conclusion