Abstract
In present study we investigated the anti-inflammatory effect of polyphenolic fraction isolated from Red cabbage Brassica oleracea Linn var. capitata f. rubra (PBO) on lipoplysacharide (LPS) stimulated HT-29 colonocytes and in rats with acetic acid induced colitis. Our results from in vitro studies demonstrated that PBO effectively attenuated the inflammatory response produced by cycloxygenase (COX), 5-lipoxygenase (5-LOX) and oxidative stress created via reactive oxygen species (ROS) and nitric oxide (NO) in LPS treated HT-29 cells. Additionally PBO down regulated the mRNA expression of inflammatory marker genes like COX-2, inducible nitric oxide (iNOS) tumor necrosis factor α (TNF-α), interleukin-6 (IL-6) in HT-29 cells. PBO at a dose of 200 mg/kg body weight was tested in treatment groups of animals (n = 6) for 14 consecutive days after induction of colitis. The colonic mucosal injury was assessed by histological examination. Moreover, PBO administration markedly increased the mucin content as evidenced in periodic acid Schiff (PAS) staining and the mucosal content of lipid peroxidation (LPO), confirms that PBO could significantly inhibit colonic mucosal damage. These results indicated that PBO exert marked anti-inflammatory effect in experimental colitis, possibly by regulating the antioxidant and inflammatory mediators.
Highlights
Ulcerative colitis (UC) is an inflammatory disease of the large bowel which is more common in the Western and Northern hemispheres; the incidence is low in Asia and the Far East
In present study we investigated the anti-inflammatory effect of polyphenolic fraction isolated from Red cabbage Brassica oleracea Linn var. capitata f. rubra (PBO) on lipoplysacharide (LPS) stimulated HT-29 colonocytes and in rats with acetic acid induced colitis
Our results from in vitro studies demonstrated that PBO effectively attenuated the inflammatory response produced by cycloxygenase (COX), 5-lipoxygenase (5-LOX) and oxidative stress created via reactive oxygen species (ROS) and nitric oxide (NO) in LPS treated HT-29 cells
Summary
Ulcerative colitis (UC) is an inflammatory disease of the large bowel which is more common in the Western and Northern hemispheres; the incidence is low in Asia and the Far East. It is slightly more common in women than in men. The major etiologic factors leading to ulcerative colitis include genetic factors, immune system reactions, and environmental factors, use of Non-Steroidal AntiInflammatory Drug’s (NSAID), low levels of antioxidants, psychological stress factors, chronic smoking, and lactose intolerance. Polyphenols isolated from plant have important role in the investigation of new safe therapeutics agents against inflammatory diseases. Red cabbage (Brassica oleracea var. capitata f. rubra), is a herbaceous, biennial, dicotyledonous flowering plant, indigenous to Mediterranean region and southwestern Europe (Shama et al, 2012)
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