Abstract
Neutral carrier-based Ca 2+ -selective microelectrodes have been examined for application in concentrated multi-ion solutions, Calculations with data from the literature and our calibration series with Ca 2+ -EGTA buffers (a convenient algorithm for their calculation is given) provide the physico-chemical conditions for determination of submicromolar concentrations of free Ca 2+ in the cytoplasm (with about 400 mM K + and 70 mM Na + ) of the marine alga Acetabularia acetabulum. The experimental results give a cytoplasmic concentration of 560 nM free Ca + corresponding to 140 nM activity
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