Abstract

The relative amount of cytoplasmic DNA in sea urchin oocytes was studied at ten successive stages of oocyte differentiation in the sea urchin, Strongylocentrotus purpuratus. The cytoplasmic DNA was selectively localized by means of 3H-actinomycin D binding on 5 µ sections of the ovaries. The specificity of the 3H-actinomycin D binding to DNA was established using DNase extracted control slides. The results show that cytoplasmic DNA increases continuously during the primary and growing oocyte stages. Although oocytes at later stages of differentiation continue to increase in volume, there is no increase in cytoplasmic DNA during these later stages. The cytoplasmic DNA observed in this study is interpreted as mitochondrial DNA.

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