Cytoplasmic competence of reconstructed GV-oocytes can be achieved with in-vitro maturation (IVM).

Abstract

Objective: Fertilization and embryo development potential of reconstructed oocytes after GV transfer may be greatly compromised by a lack of critical cytoplasmic factors. Human chorionic gonadotropins (hCG) and epidermal growth factors (EGF) have been demonstrated to induce final maturation of preantral follicles in-vitro. The aim of this investigation was to assess whether grafted GV-oocytes can be matured in-vitro to gain full competence for oocyte maturation, fertilization and embryogenesis.