Abstract
Characterization of the viral factor(s) that regulate the degree of virulence in animals and toxicity for cells in vitro, was attempted. Comparative studies, employing a large- and small-plaque variant isolated from stock lysate, were conducted on: the development of cytopathic alterations; rapidity of rupturing outer membranes of cultured cells; and efficiency for killing animals. The variant which makes large plaques is the more virulent, as judged by its capacity to kill animals and cultured cells more rapidly. Experiments with a fast-acting inhibitor, streptovitacin A, indicate that a cytotoxic principle responsible for initiating cell degeneration is a protein, possibly a viral coat protein, synthesized under the regulation of a late viral function. We have considered the mechanism whereby such a protein, once it has been synthesized, could trigger cytopathic changes in host cells by initiating a leakage of hydrolases from lysosomes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
