Cytomorphology of Gentiana kurroo: an important endangered bitter plant of temperate Himalaya

Abstract

Gentiana kurroo, a potent bitter drug plant of Indian subcontinent, is under threat due to over exploitation and destruction of natural habitat. We studied the morphophenology and chromosomes of G. kurroo on both wild and field grown plants, which is very important for proper identification, conservation and domestication. Results reveal that G. kurroo is a perennial herb, and its shoot is represented by flowering branches only. Stem is modified to rhizome. The older rhizomes split into four parts at collar region appearing to fuse together at the ends and is an important diagnostic feature for crude raw materials. Two types of leaves i.e. radical leaves at the base of the plant and cauline leaves on flowering shoot are present. Flowering occurs during September to October with 1–9 inflorescences per plant. Inflorescence is terminal monocasial cysome type. Flowers are protrandus. Anthesis starts around 7.30 am and continued till 10.0 am. Ovary is bicarpillary syncarpous unilocular. Fruit is Capsule and takes 18–20 days to mature after fertilization. Seeds are very small elliptical and 1 000 seeds weigh to 0.1275 g. Chromosomal studies made by usual squash method reveals the species is a genomic allotetraploid with n = 13. The anaphase-I segregation was normal and in none of the cells at Anaphase-I or Telophase-I could any abnormality like laggards, bridges, micronuclei etc. be observed.