Abstract
Abstract The phenomenon of centrifugal enhancement of infectivity was examined in some detial for the murine cytomegalovirus (MCV). Centrifugal enhancement (meaning that application of virus to monolayer cultures with the aid of a centrifugal field resulted in a 20- to 80-fold increase in infectious centers), was observed in mouse embryo cultures from three different strains of mice, in 3T3 cells, and in rat kidney cells. Three different strains of MCV and 20 plaque-purified strains of MCV all showed the property, as did the one strain of human cytomegalovirus examined. In contrast, herpes simplex virus type 1 and type 2 did not. Centrifugal enhancement could not be explained by increased penetration of MCV or its DNA into cells and their nuclei. Other experiments involving PFU-dose response, uv-inactivation kinetics, electron microscopy, and a variety of labeling regimes with thymidine and uridine isotopes, ruled out the presence of interfering microorganisms. It is concluded, therefore, that the property of centrifugal enhancement is an inherent property of MCV particles, and furthermore, it is suggested that some cytomegaloviruses may have a tendency to enter into a nonreplicating state in homologous fibroblasts.
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