Cytomegalovirus (CMV) IgG avidity and their relationship with immunoglobulins (IgG and IgM) levels for primary cytomegalovirus diagnosis

Abstract

In seropositive patients, HCMV disease may occur either by reactivation of a pre-existing strain or by reinfection with a new one. This study aims to assessment of HCMV antibody titers to determine the immune status and the extent to which patients are at risk of infection and measurement of antibody avidity to HCMV and its comparison with molecular diagnosis. The total IgG and IgM using nephlometry technique were measurement. The IgG levels (Mean ± SD) of control group (804.0384±173.789) showed highly significant differences (P< 0.000) among studied groups, and had elevated mean values in comparison with KD and HD patients. Also, IgM levels (Mean ± SD) showed significantly decreased (P< 0.000) in KT patients (20.685±4.787) and HD patients (21.751±6.026) in comparison with healthy control group (129.4503±35.3807). Anti-HCMV IgM, anti-HCMV IgG and anti-HCMV IgG avidity were measured using a method elctro-chemiluminescence immunoassay (ECLIA) by using cobas e411 device. A study conducted on HCMV IgM antibodies showed that there were a percentage of 4(16.66%) among the recent infections and a percentage of 3(12.5%) among cases of intermediate HCMV infection in KT group only, while the rest of the groups (17, 70.84%) did not show any recent infections with HCMV.