Abstract
Isolates of Sclerotinia sclerotiorum (Lib.) de Bary, S. trifoliorum Erikss. and S. minorJag. were studied to determine if the numbers of nuclei in mature ascospores and chromosome numbers of these fungi could be used for identification purposes. Isolates of S. minor had four nuclei in each ascospore and a haploid chromosome count of four, S. trifoliorum had four nuclei in each ascospore and a haploid chromosome count of eight, and S. sclerotiorum had two nuclei per ascospore and a haploid chromosome count of eight. Thus, consistent cytological differences were observed, supporting our earlier conclusions that S. minor, S. trifoliorum and S. sclerotiorum are distinct species.
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