Abstract
Cytology of rheumatoid synovial cells in culture. III. Significance of isolates of epithelial cell lines.
Highlights
454 Annals ofthe Rheumatic Diseases examination for the fast variant ofthe glucose-6-phosphate dehydrogenase isoenzyme. This isoenzyme is characteristic of many Negro populations and is present in HeLa cells, a line derived from a cervical cancer in a Negress, and is present in many continuous cell lines, presumably originating from contaminating HeLa cells (Gartler, 1968)
When first noticed in a culture held in a Roux flask, large areas of cells of epithelial morphology were seen in association with an intervening monolayer of fibroblasts of the type normally present in synovial membrane cultures prepared in this fashion
In the two subsequent instances the epithelial 'transformation' was observed in primary synovial fluid cultures as distinct rounded foci, just visible to the naked eye, which enlarged in diameter and in the centres of which dense piling up of cells occurred as incubation was continued
Summary
We synovial culture from a rheumatoid knee; these cells draw attention again to the dangers of inadvertent grew in suspension, were not adherent to glass, and contamination with cells, this time in the particular synthesized IgG and IgM. The general procedures in this series, we have experienced three episodes of for handling cells followed the methods of Paul (1970) Such apparent 'spontaneous transformations' in cell Chromosome preparations were made by a technique cultures from patients with rheumatoid arthritis. 454 Annals ofthe Rheumatic Diseases examination for the fast variant ofthe glucose-6-phosphate dehydrogenase isoenzyme This isoenzyme is characteristic of many Negro populations and is present in HeLa cells, a line derived from a cervical cancer in a Negress, and is present in many continuous cell lines, presumably originating from contaminating HeLa cells (Gartler, 1968). For estimation of reverse transcriptase activity, cell extracts were prepared after disruption (Coffin and Temin, 1971) and assayed by the method of Temin and Mizutani (1970)
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