Abstract

Chromosome cytology of Setaria digitata, a bovine filarial nematode has been studied by means of acetocarmine squash technique in the spermatocytes, oocytes and embryos at early cleavage. The spermatocytes showed 5 bivalents and one univalent (n=6) at metaphase-I. Six bivalents were observed in the oocytes at diakinesis of first maturation division. Diploid chromosome number of 2n=12 as well as 2n=11 was observed in embryos at early cleavage. The cytological studies indicate that the diploid chromsome number is 5A-XX in female and 5A-XO in males. Existence of XX-XO mechanism of sex determination, with male as hetergametic sex, is thus suggested in S. digitata.

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