Abstract
Penetration of Tipula irisdescent virus (TIV) in suspension-cultured cells of Estigmene acrea occurred by viropexis within 1.5 hr postinoculation (pi), followed by the uncoating of the genome in pinocytotic vesicles. Viroplasmic centers appeared in the cytoplasm of the cells by 4 hr pi and increased in size and number until the majority of the cells displayed symptoms of viral synthesis. Assembly of progeny virions was restricted to isolated pockets of “loose matrix” within the viroplasm while viral DNA accumulated in surroundings areas of higher density viroplasm. Viral release occurred by exocytosis although cell lysis could not be excluded as a possible alternative. Cytopathic effects of TIV infection included cell fusion from 7–29 hr pi, alterations in nuclear morphology, and a rapid inhibition of host-cell macromolecular systhesis as measured by radio-isotope incorporation.
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