Abstract
Cytological studies using fluorescence in situ hybridization (FISH) technique provides phylogenetical information in closely related taxa and have been widely applied for karyotyping and studying chromosomal organization and evolution in plant species. In the present study, FISH using a microsatellite sequence of (AAG)7 as the probe was performed in order to discriminate the chromosomes in four Lactuca species, i.e., L. sativa, L. serriola, L. saligna, and L. virosa. The experiment was carried out in April to September 2018 at Laboratory of Genetic and Plant Breeding of Breeding of Graduate School of Horticulture, Chiba University, Japan. Different distribution patterns of (AAG)n signals were shown on the chromosomes in the four Lactuca species studied, In L. sativa and L. serriola, FISH with (AAG)7 sequences revealed dispersed distribution patterns with one pair of bright signals, respectively. While in L. saligna and L. virosa, distinct signals with different intensities were observed in two pairs of chromosomes of L. saligna and five pairs of chromosomes of L. virosa. In conclusion, the AAG repeat signals could be used as cytogenetic landmarks for chromosome identification in Lactuca species.
Highlights
Cultivated lettuce (Lactuca sativa L.)is an economically important member of the genus Lactuca, which includes closely related wild species, L. serriola, L. saligna and L. virosa
MATERIALS AND METHODS Chromosomal study of lettuce chromosomes by fluorescence in situ hybridization (FISH) using (AAG)7 as the probe was conducted in April to September 2018 at Laboratory of Genetic and Plant Breeding of Graduate School of Horticulture, Chiba University, Japan
Images of mitotic metaphase of four Lactuca species visualized after FISH using (AAG)7 sequence as the probe are shown in Figure. 1
Summary
Cultivated lettuce (Lactuca sativa L.)is an economically important member of the genus Lactuca, which includes closely related wild species, L. serriola, L. saligna and L. virosa. Karyotype analyses were carried out to demonstrate the relationships among the cultivated lettuce and its closely related species (Haque and Godward 1985; Koopman and De Jong 1996; Lindqvist 1960; Matoba et al, 2007). Koopman et al (1993) applied chromosome banding techniques to discriminate the chromosomes of Lactuca species. Widarmi et al (2019) and Matoba et al (2007) applied FISH technique using 45S and 5SrDNAs to identify the chromosomes of Lactuca species. From these studies, it was clarified that chromosomal characteristics of FACULTY OF AGRICULTURE, UDAYANA UNIVERSITY
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