Cytological study of Сhloromonas typhlos (Chlamydomonadaceae, Chlorophyta) from North-Western Russia

Abstract

The strain of biflagellated green monad, selected from the specimen gathered in the Novgorod Region was studied by light and electron microscopy. The species was identified on the base of coincidence of the majority of specific light and ultrastructural signs revealed in both the strain SAG 26.86 of Chloromonas typhlos (Gerloff) Matsuzaki et al. and in studied strain. On LM level the cells are ellipsoid, some asymmetric, 12–17 μm long, 8–13 μm wide, with an apical, hardly distinguished hemispherical papilla flattened on the top. Chloroplast parietal, cup-shaped, thickened on one side, with big lobes, irregular incisions and fissures on the surface. These and other features are characteristic of the type and the studied strain of C. typhlos, excluding stigma observed in the studied strain only. On the ultrastructural level, large thylakoid packs, similar with chloroplast thylakoids, were observed in pyrenoids of both strains of C. typhlos. The detected pyrenoid type was never discovered in chlamydomonads earlier. It is the first record of C. typhlos for the Novgorod Region and the whole Russia, and adds the list of «bloom-forming» species. The strain was deposited in the collection of microalgae strains in the Institute of Biology of Komi Scientific Centre under the name SYKOA Ch-063-17.