CYTOLOGICAL STUDIES ON THE ADEN OHYPOPHYS IS IN RESPONSE TO CINCHOPHEN

Abstract

The author has consecutively investigated cytological changes in the adenohypophysis of the dog following oral administration of cinchophen.For the cytological manifestation, GOMORI's aldehyde-fuchsin-azan and periodic acid SCHIFF reaction were employed and differential cell count was made.Thirty minutes after administration, α-cells were somewhat enlarged and vacuolated β-cells and PAS positive cells already increased. Af ter one hour, degranulation in α-cells was found especially in apical part of the cell and granules in the β-cell were decreased with extensive vacuolation in cytoplasm. After 3 and 5 hours, α-cell degranulated progressively and diminished in its size and number. Granules in β-cells decreased and were hardly stained by fuchsin. Size of β-cells was strikingly variable. On the other hand, chromophobes, particularly granulated chromophobes, were increased. Changes in δ-cells were not distinct. After 7, 12 and 24 hours, restoration progressed, although not accomplished even after 24 hours.ACTH release after administration of cinchophen has been revealed in both previous and this, investigations, but additional hormone release seemed also not deniable from these findings.