Abstract
THREE COLLECTIONS of Aleuria species, growing in damp soil on rotting wood, were made at Hayward, Wisconsin, during August, 1931.2 One of the collections of about thirty maturing apothecia, at first noted as a variant of Aleuria wisconsinensis, according to Seaver (1928), was found, upon closer examination, to vary from the type,3 and all apothecia were fixed in alcohol-formalin-acetic.4 The preliminary studies suggested possibilities for some worth-while observations. It was decided to make a complete study of various stages in the development of the fruiting body. Especially good material for the study of the developing ascus was available, anid it was hoped that an investigation might aid in settling some of the debatable questions as to chromosome number and behavior. Sections of apothecia, mostly seven microns thick, were stained in the following ways: At first Flemming's triple stain was used and proved unsatisfactory. Other stains were tried: Heidenhain's haematoxylin, counterstained with fast green, light green, cotton blue, or Orange G; Delafield's haematoxylin with the same counterstains, also dilute safranin; and aceto-carmine with Erlich's haematoxylin. Aceto-carmine preparations proved unsatisfactory. Heidenhain's haematoxylin counterstained with dilute safranin was used to good advantage; but the best preparations were, obtained with Delafield's haematoxylin and dilute safranin. Observations were made with a Bausch and Lomb 1/16 achromatic oil-immersion objective, and all drawings were made with the aid of a Spencer camera lucida so as to give a magnification of about twenty-one hundred. In reproduction these have been reduced one-fifth. OBSERVATIONS.-The largest apothecium studied was 4 mm. in diameter, the smallest less than 1 mm.; yet the size of the apothecium did not indicate the stages to be fouind within. Even in the smallest, abundant ascogenous hyphae and asci in all stages of development were present and, in some cases, stages of ascogonia. In the youngest apothecia, the
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