Cytological investigations on cultures of normal human blood

Abstract

1. In the cultures of leucocytes of normal human blood under definite conditions of cultivation there appear periods of mass multiplication of cells in mitotic fashion. 2. The mass multiplication is observed in the polyblasts (macrophages) at the stage of their mutation into stable cells, and in the fibrocytes originating in haemocultures from polyblasts. 3. For obtaining the maximal quantity of mitoses the best time for fixation is the second 24 hours after the renewal of the medium, this latter being performed not later than the end of the fourth 24 hours after the planting of the culture. 4. After appropriate fixation, the figures of divisions may be used for karyological analysis. 5. The method proposed enlarges the possibilities of the investigation of the human karyotype, and enables us to undertake the karyological investigation of any anthropogenetical or medical phenomena.