Abstract
Twenty-eight different agents were tested for their ability to activate the production of chromosomal aberrations in the root-tips of Vicia faba by the light-acridine orange system. Of these agents only sodium nitrite and the phenylnitrosamines diphenylnitrosamine and N-methylphenylnitrosamine were active. The effect of methylphenylnitrosamine in the light-acridine orange system was compared with that of cupferron. Oxygen was found to counteract the effects of both these phenylnitrosamines. The effect of methylphenylnitrosamine was independent of the pH of the treatment solution, whereas the effect of cupferron decreased when the pH increased. The nature and mode of action of the active agents is discussed. Nitric oxide and cupferron were not able to replace oxygen in the photodynamic inactivation of coliphage T3 in the presence of acridine orange.
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