Abstract

To trace the interrelationships and for identification and characterisation of diversity among four species of medicinally important plant Bauhinia viz. B. acuminata, B. purpurea, B. racemosa, and B. variegata belonging to sub-family Caesalpiniaceae, cytological study with respect to chromosome number, somatic chromosome length of component arms of chromosome, T. F% and chromosome type and seed protein variation were investigated. Results showed that the three species have chromosome number 2n=28, the value of the total chromatin lengths was lowest (53.48µ) in B. racemosa and highest (57.32µ) in B. purpurea while in B. acuminata this value was 56.78µ suggesting a close relationship between B.purpurea and B. acuminata. The T.F% values of B. acuminata and B. purpurea stand close to each other. Beside the common bands among the studied taxa, 7.37 kDa, 31.85 kDa, 41.56 kDa, 54.854 kDa and 261.143 kDa proteins were found to be common in B. acuminata and B. purpurea and 261.143 kDa protein was found common in all the four species. Maximum genetic affinities were observed between B. acuminata x B. purpurea (45.45%), while minimum between and B. racemosa and B. variegata(20%). On the molecular level, the present study gave the results with wide variations in their band numbers. Maximum number of protein bands (11 bands) was observed in B. racemosa, while minimum (7 bands) in B. purpurea and B. variegata.

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