Cytological changes related to maturation of the organ of Corti and opening of Corti's tunnel

Abstract

Maturation of the organ of Corti in the gerbil was analyzed between 2 and 16 days after birth (DAB) by electron microscopy and immunostaining for β-tubulin. At 2 DAB, the organ of Corti consisted of stratified epithelium bearing immature sensory hair cells (HCs) and supporting cells. Maturation of OHCs and Deiters cells progressed in a medial-to-lateral direction and cytoskeletal development in inner pillar cells preceded that in outer pillar cells at the single location studied along the frequency-place map. Pillar cell differentiation progressed through a unique stage characterized by the appearance and stratification of structural features apparently concerned with opening of Corti's tunnel and subsequently showed other structural changes related to maturity toward the adult form. Development of the microtubule cytoskeleton occurred first in the cell's apex and proceeded basally. Ruffling of a middle region of the cell surface by microvilli appeared to promote separation between inner and outer pillar cells and initiate tunnel opening at 4 DAB. Proliferation of distended cisternae of granular reticulum evidenced proteinaceous secretion by these cells between 4 and 8 DAB. Subsequent tunnel expansion at about 14 DAB coincided with appearance in outer pillar cells of tubulocisternal endoplasmic reticulum and associated Golgi complexes that are thought to mediate fluid and ion secretion. Sixteen days postnatally after disappearance of granular and tubulocisternal reticula and Golgi complexes and at the time of clearing of tunnel fluid, lysosomes interpreted as mediating catabolism of endocytosed protein congregated beneath the apicala nd apicolateral plasmalemmae of inner pillar cells. As with pillar cells, development of the microtubule system in Deiters cells proceeded from the cell's apex to base. Following differentiation of their microtubule system by 8 DAB, Deiters cells showed expansion of Golgi cisternae between 10 and 15 DAB and development of tubulocisternal endoplasmic reticulum at 15 DAB. Hair cells possessed abundant, distinctively large mitochondria from 4 to 10 DAB. The subsurface cisternae matured earlier in medial as opposed to lateral outer hair cells. Vesicles budding from underlying cisternae appeared associated with development of subsurface cisternae and at 16 DAB were still observed in third row but not in more mature first row HCs.