Abstract
SummaryShoot tips of strawberry (Fragaria vesca F. ananassa) cultivar ‘Heilongjiang No.1’ were successfully preserved in vitro using a slow-growth culture method. All shoot tips survived 1 year of storage and showed an increase of 1.56 ± 0.08 cm in shoot length. In addition, eight single-bud-derived sibling lines were established for genetic analysis. Although cytological examination detected chromosomal variations in plants recovered from slow-growth, their ploidy level was similar to the control. Amplified fragment length polymorphism (AFLP) analysis with 16 primer combinations did not detected any change in AFLP patterns between controls and strawberry plants recovered from slow-growth culture. However, methylation-sensitive amplified polymorphism (MSAP) analyses indicated that slow-growth storage induced changes in DNA methylation and DNA de-methylation patterns, while no de novo methylation was found.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: The Journal of Horticultural Science and Biotechnology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
