Cytolipin H-specific antibodies obtained with a synthetic polypeptide conjugate.

Abstract

Antibodies with specificity directed towards the naturally occurring glycolipid, cytolipin H, were elicited by immunization of rabbits with a synthetic antigen. The antigen was a conjugate consisting of a linear copolymer of alanine, glutamic acid and tyrosine, to which lactosyl sphingosine groups were attached. The specificity of the antibodies obtained, as seen from both cross‐precipitation reactions with the parent copolymer and a related conjugate (galactosyl‐sphingosyl‐copolymer), and from inhibition experiments with lactose and lipid derivatives, was towards a region which included — besides the lactose moiety — the lipid part of the molecule as well.