Cytokinetic considerations of murine neuroblastoma (C1300) as a screening model for the childhood disease

Abstract

Kinetic studies are presented for the Murine Neuroblastoma C 1300 system. Animals receiving subcutaneous inoculations of 10 6 cells develop a palpable tumor with a mean diameter of 1·3 cm in 10 days. The growth characteristics of palpable disease are consistent with a Gompertzian curve distribution; the doubling time of palpable disease is 10 days. Autoradiographic studies demonstrated a DNA synthesis time of 4·52 hr and a total generation time of 14·1 hr. These considerations and the sensitivity of the model to cell cycle nonspecific agents suggest that the C 1300 system deserves further study as a potential model of the human disease.