Cytokine-induced killer cells and supernatant on the role of klebsiella pneumoniae

Abstract

Objective To investigate the ability of cytokine-induced killer (CIK) cells and supernatant against klebsiella pneumoniae in vitro.Methods CIK cells were prepared in vitro.The phenotype of CIK cells was analyzed by flow cytometer in 1,7,14,21 day.Both microdilution method was used to detect the minimum inhibitory concentration (MIC) values of CIK cells against klebsiella pneumoniae (final inoculation concentration of bacteria was 1.0 × 105 cfu/ml,target ratio was 40∶ 1,20∶ 1,10∶ 1,5∶1),the supernatant,and amikacin (AK),piperacillin (PIPC) joint after,to determine their antimicrobial activity in vitro.Results In the culture section 14-21 day,CD3 + CD56 + cell phenotype proportion remained at a high level more than 45％.Absorbance (A) values for each test showed that different concentrations of CIK cells killing effect were no statistically significant (F =3.065,P ＞ 0.05) at 24 h.The supernatant group showed no significant bacterial growth.The MIC values of amikacin (AK),piperacillin (PIPC) were 2,512 mg/L respectively.CIK cells respectively,and AK,PIPC joint after,the latter failed to reduce the MIC values,but the supernatant reduced the MIC values of both to the minimize inoculum concentrations of 0.25 mg/L and 64.00 mg/L.Conclusion CIK cells showed no direct killing effect against klebsiella pneumoniae in vitro,and no synergistic reaction combined with antibiotics,but the supernatant has antibacterial effects to klebsiella pneumoniae. Key words: Cytokine-induced killer cells; Klebsiella pneumoniae; Minimum inhibitory concentration