Abstract
Systems of antigen delivery into antigen-presenting cells represent an important novel strategy in chicken vaccine development. In this study, we verified the ability of Rous sarcoma virus (RSV) antigens fused with streptavidin to be targeted by specific biotinylated monoclonal antibody (anti-CD205) into dendritic cells and induce virus-specific protective immunity. The method was tested in four congenic lines of chickens that are either resistant or susceptible to the progressive growth of RSV-induced tumors. Our analyses confirmed that the biot-anti-CD205-SA-FITC complex was internalized by chicken splenocytes. In the cytokine expression profile, several significant differences were evident between RSV-challenged progressor and regressor chicken lines. A significant up-regulation of IL-2, IL-12, IL-15, and IL-18 expression was detected in immunized chickens of both regressor and progressor groups. Of these cytokines, IL-2 and IL-12 were most up-regulated 14 days post-challenge (dpc), while IL-15 and IL-18 were most up-regulated at 28 dpc. On the contrary, IL-10 expression was significantly down-regulated in all immunized groups of progressor chickens at 14 dpc. We detected significant up-regulation of IL-17 in the group of immunized progressors. LITAF down-regulation with iNOS up-regulation was especially observed in the progressor group of immunized chickens that developed large tumors. Based on the increased expression of cytokines specific for activated dendritic cells, we conclude that our system is able to induce partial stimulation of specific cell types involved in cell-mediated immunity.
Highlights
Generation of de novo adaptive responses, including responses to vaccines, is primarily elicited by dendritic cells (DC), specialized leukocytes adapted for antigen capture, processing and presentation to T lymphocytes
SA‐Rous sarcoma virus (RSV) fusion proteins The complete sequence of the RSV antigens v-src, env, pol and gag were fused to the N- and C-terminus of the tetramerization core of streptavidin
For the first time in the chicken, we verified the ability of fusion complexes of RSV antigens with SA to target specific biotinylated monoclonal antibody
Summary
Generation of de novo adaptive responses, including responses to vaccines, is primarily elicited by dendritic cells (DC), specialized leukocytes adapted for antigen capture, processing and presentation to T lymphocytes. Knowledge of these cells in a target species is crucial in finding the most effective means of vaccination. DC express, among other, the CD205 (Ly75, DEC205) molecule that functions as an endocytic receptor involved in the uptake of extracellular antigens. DC could be targeted with antigen-conjugated monoclonal antibodies specific for CD205, which are efficiently internalized, processed in the endosomal compartment, and presented to both major histocompatibility complex I (MHC I) and MHC II molecules [5]
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