Cytokine profile of patients with severe hemorrhagic fever complicated with acute renal failure

Abstract

Objective: to determine the cytokine profile in blood serum in patients with severe hemorrhagic fever complicated by acute renal failure. Materials and methods. The examined persons were divided into a control group – 46 people (healthy), a group of patients with an average course of the disease – 35 people, and a group of patients with severe severity, complicated by acute renal failure – 38 people. Blood was taken from the subjects for the study of cytokines. The determination of cytokines was carried out by enzyme immunoassay using test kits in accordance with the manufacturer’s instructions. Results. In patients with hemorrhagic fever, both with moderate and severe, there was an increase in interleukins IL-1β, IL-6, IL-10 compared with the control group. However, the values of these interleukins were significantly higher in patients with severe hemorrhagic fever complicated by acute renal failure compared with the group of patients with moderate severity. The values of IL-8, IFN-γ and SDF-1α changed only in patients with severe course. At the same time, there was an increase in the expression of IL-8 and a decrease in IFN-γ compared with the control group. IL-8 and IFN-γ did not change statistically in patients who had just been admitted to the hospital, as well as in patients with an average degree of the disease. A change in the SDF-1α chemokine also turned out to be specific for patients with a severe course of the disease: its values increased in comparison with the control group. No significant changes in SDF-1α were detected in patients who had just been admitted to the hospital, as well as in the group of patients with moderate severity of the disease. Conclusion. The results obtained indicate the diagnostic value of cytokine determination in severe hemorrhagic fever complicated by acute renal failure. Changes in the parameters of some cytokines, detected only in the profile of patients with severe severity, require further research to clarify their role in the pathogenesis of hemorrhagic fever, and also open up opportunities for considering them as biomarkers.