Cytokine profile in tear and aqueous humor of primary open‐angle glaucoma patients as a prognostic factor for trabeculectomy outcome

Abstract

AbstractPurposeTo evaluate the cytokine profile in tear and aqueous humor before trabeculectomy and correlate cytokine levels with the surgical outcome.MethodsProspective study. Twenty‐nine patients with primary open‐angle glaucoma undergoing primary trabeculectomy were included. Levels of 27 cytokines were measure in tear an aqueous humor using the Bio‐Plex Pro Human Cytokine 27‐Plex Immunoassay kit (Bio‐Rad Laboratories, Hercules, CA, USA).Results29 patients who underwent trabeculectomy were included and their first‐year follow‐up visits were recorded. Mean age was 76.0 ± 7.0 years (range 56–84), mean intraocular pressure was 18.2 ± 3.6 mmHg and mean number of topical medications was 2.3 ± 0.9. At the one‐year visit, 5 patients were classified as surgical failure. In aqueous humor, preoperative cytokine levels of regulated on activation normal T cell expressed and secreted (RANTES) were significantly higher in those patients with surgical failure at one year. IL‐8 in tear and interferon gamma‐induced protein (IP‐10) in aqueous humor correlated positively with one‐year IOP reduction. No statistically significant correlations were found with changes in visual field mean defect or global peripapillary retinal nerve fiber layer thickness (all, p > 0.05).ConclusionsPreoperative RANTES levels in aqueous humor as well as other cytokines could serve as useful biomarkers for trabeculectomy outcome.