Abstract
Oxidized low-density lipoprotein (OxLDL) modulates the vascular smooth muscle cells (VSMCs) functions. Apoptosis of VSMCs occurs in vivo under both physiological and pathological conditions. However the fate of internalized apoptotic cells, the rate of intercellular degradation, and the consequences of these processes to VSMC biology are unknown. Ray-biotech rat cytokine protein microarrays were used to profile the effects of the pro-atherogenic molecule such as OxLDL on rat aortic smooth muscle cells (rVSMCs). Uptake studies with 125I-OxLDL shows that OxLDL level increases in a dose dependent manner up to a maximum level of 100μg/2x109 cells/ml. Furthermore, OxLDL causes both cell proliferation at lower dose (50 μg/ml) and cellular apoptosis at higher dose (100 μg/ml). Cytokine protein microarrays detected 15 proteins in cell lysates and OxLDL differentially regulated 13 of those. OxLDL has bi-directional effect on MCP-1, VEGF, TNFá, and IL-1â. Furthermore, OxLDL stimulates dose dependently the production of TNFá, NGF. These findings reveal the major pattern of OxLDL-induced effects on the rat aortic smooth muscle cells functions and also demonstrate that protein chip-based microarrays could be a useful proteomic tool to profile disease-related states of muscles. (Supported by grants NIH 5T32HL07809-10 and DAMD17-03-2-0054)
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