Abstract
Methods ST samples from the inflamed knee joint of 30 patients fulfilling the CASPAR criteria for PsA were immunostained with CD3 (T cell), CD20 (B-cell) and MECA-79 (high endothelial vessels). SLN was defined by lymphoid aggregates grade 2-3 (>6 radial of B and T cells), T-B cell segregation and MECA-79 positive vessels. mRNA was also extracted from ST samples of patients and 2 controls without synovial inflammation, and measured by TaqMan. Relative changes in gene expression were assessed by the delta-delta CT method using GAPDH as an endogenous control gene. The following cytokines and receptors were analyzed: CCR7, Lymphotoxin (LT)-beta (b), IL-7, IL-10, IL-1b, IL-6, TNF-a, IL-17 A, IL-21, IL-22 and IL-23. Clinical and biological data were collected at inclusion and at the end of follow-up.
Highlights
To analyze the expression of cytokines in synovial tissue (ST) of patients with psoriatic arthritis (PsA) and their relationship with synovial lymphoid neogenesis (SLN) and disease activity at baseline, and with erosive disease at the end of follow-up
ST samples from the inflamed knee joint of 30 patients fulfilling the CASPAR criteria for PsA were immunostained with CD3 (T cell), CD20 (B-cell) and MECA-79
SLN was defined by lymphoid aggregates grade 2-3 (>6 radial of B and T cells), T-B cell segregation and MECA-79 positive vessels
Summary
Cytokine expression in synovial tissue of psoriatic arthritis and its relationship with lymphoid neogenesis, disease activity and erosive disease: a longitudinal study. From 5th European Workshop on Immune-Mediated Inflammatory Diseases Sitges-Barcelona, Spain. From 5th European Workshop on Immune-Mediated Inflammatory Diseases Sitges-Barcelona, Spain. 1-3 December 2010
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