Cytokine and Chemokine Responses to Selected Early Secreted Antigenic Target-6 and Culture Filtrate Protein-10 Peptides in Tuberculosis

Abstract

Cytokine [tumor necrosis factor-α, interleukin-2 (IL-2), IL-4] and chemokine [regulated upon activation normal T cell expressed and secreted (RANTES), monocyte chemoattractant protein-1] responses to selected early secreted antigenic target-6 (ESAT-6) and culture filtrate protein-10 (CFP-10) peptides were studied in healthy household contacts and patients with pulmonary tuberculosis (PTB). It was observed that Th1 cytokines and chemokine RANTES positive T cells were elevated in response to the peptides Esp1, Esp6, Cfp6, and Cfp8 in healthy household contacts. IL-4 positive T cells were enhanced by Esp1 and Esp6 in PTB. Monocyte chemoattractant protein-1 positive monocytes increased in response to the peptides Esp1, Esp6, Cfp8, and Cfp9 in PTB. These peptides deserve attention for further immune studies.