Abstract
Cytogenetical investigations on a spontaneous male sterile mutant isolated from the inbred lines of white flowered variety of Petunia axillaris (Lam.) B.S.P., revealed a digenic pattern of inheritance of the mutant character. The original mutant as well as those obtained subsequently in F2 and F3 generations showed unimpaired female fertility and resembled in morphology the male fertile sibs, except for the small, shrivelled empty anthers.Microsporogenesis in the sterile paints was apparently normal upto the liberation of microspores from the tetrads, which degenerated subsequently resulting in complete absence of pollen grains in the anthers. In addition, male sterility was found to be invariably associated with atypical behaviour of tapetum, characterised by prolonged nuclear divisions and untimely degeneration as a result of conversion from glandular to periplasmodial type.
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