Abstract
A bovine XY female in Holstein-Friesian heifer, which appeared as female with uterus and ovaries but did not show the estrus until 23 months old after the birth, was cytogenetically and molecularbiologically examined. As results of chromosome analysis, leucocyte and fibroblasts from skin, spleen and kidney examined had only metaphase plates with 60, XY. From these results and the clinical characteristics, this case was clearly diagnosed as a pure XY female. It was ascertained that the two genes, ZFY and AMG gene which located on the short arm of the Y chromosome (Yp) were detected in normal bulls and a XY female, but were not detected in normal cow, mother cow and half-sib heifer by Southern blotting.
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