CYTOGENETIC VARIABILITY OF HYBRID FORMS OF MOUNTAIN ASH AND SIBERIAN ROWAN (SORBUS AUCUPARIA L., 1753 × SORBUS SIBIRICA HEDL., 1901) UNDER ANTHROPOGENIC LOAD

Abstract

The influence of anthropogenic effects on the cytogenetic indicators of hybrids of mountain ash and Siberian rowan (Sorbus aucuparia L., 1753 × Sorbus sibirica Hedl., 1901) was studied. The study was conducted in the summer of 2018 in the south of the Tyumen region, in the cities of Ishim and Tyumen. For cytogenetic analysis, young mountain ash leaves were collected and fixed with Clark's reagent. The material was stained in a routine manner with aceto-Orsein. In the course of work, about 200 temporary preparations were prepared. It was found that in the studied areas of the cities of Tyumen and Ishim, the frequency of occurrence of mitosis anomalies was statistically significantly higher compared to the control. In total, 5 types of cytogenetic anomalies were revealed in mountain ash: fragments, micronuclei, single, multiple bridges, and abnormal forms of the nucleus. Moreover, such anomalies as micronuclei and fragments are most frequently encountered, and the anomalous forms of the nucleus are the least. In Tyumen, the frequency of occurrence of cytogenetic anomalies is 1.4 times higher than in Ishim, which indicates a higher degree of anthropogenic load on the territory of this city.