Cytogenetic Study in a Mutant of Triatoma infestans (Hemiptera: Reduviidae) Carrying a Spontaneous Autosomal Fusion and an Extra Chromosome

Abstract

Triatomainfestans (2n = 20 A + XY, male) is a blood-sucking bug and the most important vector of Chagas disease in the Southern Cone countries. A cytogenetic analysis of 14 individuals from the Argentine Gran Chaco has revealed the presence of a naturally heterozygous for an autosomal fusion. The fusion heterozygote (2n = 19 A + 1 extra chromosome + XY, male) presented an autosomal trivalent, 8 bivalents, the X and Y sex univalents, and a minute extra chromosome at meiosis I. The autosomal trivalent divided equationally at first anaphase. At metaphase II, cells had 8 autosomes, X and Y sex chromosomes, and an autosomal pseudo-trivalent composed by 3 different-sized chromatids. The orientation of this pseudo-trivalent led to a reductional segregation. The meiotic behaviour of this new chromosome complement was highly regular. The extra chromosome did not affect the segregation of autosomes and sex chromosomes during both meiotic divisions. We propose that the extra chromosome was originated as a product of an autosomal fusion, and it might become a B chromosome. Many authors suggest that karyotype evolution in Heteroptera has proceeded mainly by fusions and fragmentations. The fact that this rearrangement has been found in a natural population of T. infestans and that it shows a regular meiotic behaviour seems to support the suggested hypothesis.