Abstract

Abstract Objectives The high salinization stress to seedling is the substantial ecological problem in the ongoing era. It negatively influences the growth that retard mitotic division by enhancing aberrations in nuclear chromatin. In the light of these views, the current work was designed to investigate the response of Vigna seedlings root tip cells to the presence of NaCl ions. Materials and methods NM-92 and NM19-19 seeds were imbibed separately in distilled water for 24 h and allowed to grow into 0, 50, 150, 250, and 350 mM NaCl solution for 24 h. Excised root tips were stained, and slides were scored at 100× objective for the mitotic index (MI) and chromosomal aberrations. Results Our data demonstrated that as NaCl molarity increased, the MI was declined along with various chromatin abnormalities. The 150 mM of NaCl showed more lagging (69%) of chromosomes during anaphase in NM19-19. The highest stickiness at metaphase stage (68%) was found in 250 mM NaCl in variety NM19-19. However, both varieties were differed non-significantly for c-mitosis that was recorded 99% at 350 mM NaCl concentration. Conclusions The NaCl ions toxicity induced various cytological anomalies in seedling roots that adversely affect the growth of Vigna seedlings.

Highlights

  • Materials and methodsThe soil salinization is the most important abiotic stress in the barren zone of land [1]

  • In the light of these views, the current work was designed to investigate the response of Vigna seedlings root tip cells to the presence of NaCl ions

  • Excised root tips were stained, and slides were scored at 100× objective for the mitotic index (MI) and chromosomal aberrations

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Summary

Introduction

Materials and methodsThe soil salinization is the most important abiotic stress in the barren zone of land [1]. In which sodium chloride will be standout amongst the salts in nature and has a dangerous impact due to its superior solubility [1]. It badly devastates seedlings biochemistry and inducing ionic stress [3, 4] by storing Na+ & Clions in the cytosol. These ionic toxicities distort the double helix structure of DNA and orientation of chromosome at metaphase that caused chromosome abnormalities like stickiness in chromosomes, C-mitosis, lagged and disturbed anaphase [3, 5]. No hazardous compound hinders the development of crops besides salt stress on the earth scale [6]

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