Abstract
Chromosome count is the only direct way to determine the number of chromosomes of a species. This study is often considered trivial that seldom described and discussed in detail. Therefore, it is inevitable that the chromosome count protocol should be revised and revisited before it becomes obliterated. In the present study, we encountered challenges in obtaining a clear micrograph for the chromosome count of active mitotic cells of Neolamarckia cadamba (Roxb.) Bosser (Rubiaceae) root tips. Several obstacles were determined through micrograph observation, such as existing unwanted particles in cells, poor chromosome staining and chromosome clumping. To overcome these, root tip types, staining methodologies, squashing methods were among the factors assessed to obtain clear micrographs. The chromosome counts of N. cadamba under optimized procedure showed 2n = 44 chromosomes. We also apply digital technology in chromosome counts, such as online databases and graphic software that are open source and freely accessible to the public. Only basic laboratory equipment and chemicals were used throughout the study, thus making this study economical and applicable in a basic laboratory. The availability of online digital software and databases provide open-source platforms that will ease the efforts in chromosome count.
Highlights
Neolamarckia cadamba (Roxb.) Bosser (Rubiaceae) is a large, deciduous and fast-growing tree species, and guarantee early economic return within 8 to 10 years (Ho et al, 2014; Pang et al, 2015; Tchin et al, 2018 a,b)
We described the optimization and automation procedures for the chromosome counts in N. cadamba and ascertained that the previous colchicine induced mutation study was sufficient to induce polyploidy (Eng et al, 2021)
The micrograph captured in this study showed well-spread and visibly distinct chromosomes with a clear background in the nucleus
Summary
Neolamarckia cadamba (Roxb.) Bosser (Rubiaceae) is a large, deciduous and fast-growing tree species, and guarantee early economic return within 8 to 10 years (Ho et al, 2014; Pang et al, 2015; Tchin et al, 2018 a,b). It is one of the best sources of raw material for the plywood industry, besides pulp, paper production and medicinal purposes. From Volume 13, Issue 1, 2021, Notulae Scientia Biologicae journal uses article numbers in place of the traditional method of continuous pagination through the volume. The journal will continue to appear quarterly, as before, with four annual numbers
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