Abstract
The cytogenetic characterization of the L5178Y TK +/− 3.7.2C mouse lymphoma cell line was carried out, utilizing G-banded metaphase chromosomes, to provide a karyotypic basis for the precise delineation of induced rearrangements in TK −/− mutants. Band-pattern measurements were used to construct ideograms which represent the position, number, size and staining intensity of the chromosome bands. The TK +/− 3.7.2C cell line has been shown to provide quantitation of forward mutations induced at the autosomal thymidine kinase (TK) locus in this cell line. Chromosome analysis of the TK +/− 3.7.2C cell line and derived TK −/− mutants has become important in demonstrating that the TK +/− → TK −/− assay may detect and distinguish between chromosomal events and smaller, perhaps point-mutation, events in mutant colonies.
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