Cytogenetic and syntenic assignment of the bovine &amp;lt;i&amp;gt;platelet-activating factor receptor (PTAFR)&amp;lt;/i&amp;gt; to cattle chromosome 2 (Brief report)

T Goldammer,P Schmidt,R Weikard

doi:10.5194/aab-52-448-2009

Abstract

Abstract. The platelet-activating factor receptor (PTAFR) encoding gene, also known as PAFR or PAFr, belongs to the rhodopsin gene family. The receptor binds the platelet-activating factor (PAF) that has been implicated as a mediator in diverse pathologic processes. In cattle, PTAFR is associated to the reproduction process and is described as a receptor that is involved in inflammatory-like processes of the uterus associated with increased vascular permeability (TIEMANN et al. 2005). The gene sequence was recently annotated on Bos taurus (BTA) chromosome 2 at 129.4 megabases in NCBI Bos taurus build Btau_4.0. The presented data confirm this annotation by independent physical mapping methods and anchor the corresponding DNA segment to the chromosome. PTAFR was assigned by fluorescence in situ hybridization (FISH) and somatic hybrid cell (SHC) mapping.

Highlights

The platelet-activating factor receptor (PTAFR) encoding gene, known as PAFR or PAFr, belongs to the rhodopsin gene family
DNA sequencing of the PCR product was performed to verify the amplification of the bovine PTAFR DNA sequence
The PCR reaction in each cell line of the somatic hybrid cell (SHC) panel was performed in a final volume of 10 μl with 100 ng DNA, 10 pmol of each primer, 200 μM dNTPs, 0.15 U Taq DNA Polymerase (Promega), and standard enzyme buffer conditions

Summary

Introduction

The platelet-activating factor receptor (PTAFR) encoding gene, known as PAFR or PAFr, belongs to the rhodopsin gene family. Pools of the 5× genome covering bovine BAC library BBI_B750 (ZHU et al 1999) were received from the former Resource Center of the German Human Genome Project (RZPD, Berlin, Germany) and screened by PCR to obtain a genomic DNA fragment specific bovine specific PTAFR. DNA sequencing of the PCR product was performed to verify the amplification of the bovine PTAFR DNA sequence. Genome database search using the NCBI BLAST tool identified similarity between the bovine PCR product and human genome data.

Results

Conclusion